منابع مشابه
GENOTYPING OF PANDHARPURI BUFFALO FOR k-CASEIN USING PCR -RFLP
The objective of the study was to genotype Pandharpuri buffalo breeds for k-casein type by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A 379-bp k-casein gene fragment was amplified using primers K1 (5’CAC GTC ACC CAC ACC CAC ATT TAT C-3’) and K2 (5’-TAA TTA GCC CAT TTC GCC TTC TCT GT-3’). DNA from 20 Pandharpuri buffaloes were extracted by phenol chloroform me...
متن کاملGenotyping of the Holstein-Friesian crossbred cattle for gene using PCR-RFLP CD18
Bovine Leucocyte Adhesion Deficiency (BLAD) is a Holstein-Friesian HF) specific autosomal recessively inherited disorder characterized by recurrent pneumonia, ulcerative and granulomatous stomatitis, enteritis, periodontitis, loss of teeth, delayed wound healing, persistent neutrophilia and death at an early age [1]. BLAD is a disease characterized by a reduced expression of the adhesion molecu...
متن کاملGenotypic Analysis of Staphylococcus aureus Coagulase Gene using PCR-RFLP analysis
ABSTRACT Background and Objectives: Determining the genetic relationship between S. aureus isolates is important for epidemiological surveillance and control of infections caused by this bacterium. The present study was conducted to determine polymorphisms of coagulase gene (coa) among S. aureus isolates from pastry and cheese samples usi...
متن کاملGenetic Similarity Among the Three Egyptian Water Buffalo Flocks Using RAPD-PCR and PCR-RFLP Techniques
Genetic similarity and polymorphisms among the three Egyptian water buffalo flocks (Beheiry, Minoufy and Saidy) were studied using both random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) techniques. Ten random primers were used to amplify DNA fragments in these three flocks. RAPD patterns with a level of polymorphism were detected among flocks. The resul...
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ژورنال
عنوان ژورنال: Journal of Animal Health and Production
سال: 2014
ISSN: 2308-2801
DOI: 10.14737/journal.jahp/2014/2.1.5.7